{"id":1661,"date":"2018-06-29T14:12:52","date_gmt":"2018-06-29T12:12:52","guid":{"rendered":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/?p=1661"},"modified":"2024-08-20T17:23:57","modified_gmt":"2024-08-20T15:23:57","slug":"nanoscale-distance-measurements-in-epr-and-fluorescence-spectroscopies-august-28-29-2018","status":"publish","type":"post","link":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/2018\/06\/nanoscale-distance-measurements-in-epr-and-fluorescence-spectroscopies-august-28-29-2018\/","title":{"rendered":"ATM &#8211; Nanoscale Distance Measurements in EPR and Fluorescence Spectroscopies (August 28-29, 2018)"},"content":{"rendered":"<h2>Aims<\/h2>\n<p class=\"Standard\">This module was suitable for doctoral students in chemistry, physics, biophysics, and related fields. Some background in quantum mechanics was<i>&nbsp;advisable <\/i>but was not a direct prerequisite. It introduced the basic theoretical and technical concepts necessary for an understanding of electron paramagnetic resonance and fluorescence in general and especially emphasises the EPR and fluorescence methods for measuring distances on the nanometer scale.<\/p>\n<h2>Contents<\/h2>\n<p class=\"Standard\">Each day of the two-day program started with a series of lectures in the morning covering the theoretical knowledge by basic lectures and the discussion of applications and problems. It was followed by hands-on experiments on instructive examples during the afternoon.<!--more--><\/p>\n<h4>Lectures:<\/h4>\n<ul>\n<li>basic principles of magnetic resonance, technical spectrometer set-up, chemistry of spin labeling and spin probing, pulse EPR spectroscopy, data processing\/analysis, applications to (bio-)polymers<\/li>\n<li>basic principles of single-molecule and time-resolved fluorescence spectroscopy and single-molecule FRET, including methods of fluorescence labelling, microscope set-up, single-molecule detection and data analysis<\/li>\n<\/ul>\n<h4>Hands-on lab course:<\/h4>\n<div class=\"GroupWiseMessageBody\">\n<ul>\n<li>Sample preparation and CW EPR spectroscopy; guided experiments in pulse EPR spectroscopy; applications and analysis for distance measurements<\/li>\n<li>guided single-molecule and time-resolved FRET experiments of proteins in solution, data analysis for intramolecular distance measurements<\/li>\n<\/ul>\n<h4>Recommendations for literature<\/h4>\n<ul>\n<li>A. Weil, J. R. Bolton, Electron Paramagnetic Resonance: elementary theory and practical applications. John Wiley &amp; Sons Inc.: Hoboken, New Jersey, 2007.<\/li>\n<li>Schweiger, G. Jeschke, <em>Principles of pulse electron paramagnetic resonance. <\/em>Oxford University Press Inc.: Oxford, <strong>2001<\/strong>.<\/li>\n<li>Jeschke, <em>Emerging Top Life Sci <\/em><strong>2018<\/strong>, <em>2<\/em>, 9\u201318<\/li>\n<li>J. N. Junk, H. W. Spiess, D. Hinderberger, <em>Angew Chem Int Ed <\/em><strong>2010<\/strong>,<em>49<\/em>, 8755\u20138759.<\/li>\n<li>Lakowicz, J.R. Principles of fluorescence spectroscopy, Springer, 2006<\/li>\n<li>Jammeson D.M.. Perspectives on Fluorescence, Springer, 2016<\/li>\n<li>Dimura M., et al. Quantitative FRET studies and integrative modeling unravel the structure and dynamics of biomolecular systems, Curt Opin Struct Biol 40: 163-185 (2016)<\/li>\n<\/ul>\n<\/div>\n<h2>Terms and Applications<\/h2>\n<p><strong>Fluorescence at August 28<\/strong>,&nbsp;9:30 \u2013 17:00<br \/>\nVenue:<br \/>\nMartin Luther University Halle-Wittenberg,<br \/>\nInstitute of Biochemistry and Biotechnology<br \/>\nKurt-Mothes-Str. 3,<br \/>\nSR: 100.1<\/p>\n<p><strong>EPR at August 29<\/strong>,&nbsp;9:00 \u2013 16:00<br \/>\nVenue:<br \/>\nMartin Luther University Halle-Wittenberg,<br \/>\nInstitute of Chemistry,<br \/>\nVon-Danckelmann-Platz 4,<br \/>\nSR: 1.27<\/p>\n<p>Group size: maximal 10<br \/>\nLanguage: English<\/p>\n<h2>Instructor(s)<\/h2>\n<p>Dr.&nbsp;Maria Ott, &nbsp;Prof. Dr. Dariush Hinderberger<\/p>\n<h2>Registration complete<\/h2>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Aims This module was suitable for doctoral students in chemistry, physics, biophysics, and related fields. Some background in &hellip; <a href=\"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/2018\/06\/nanoscale-distance-measurements-in-epr-and-fluorescence-spectroscopies-august-28-29-2018\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;ATM &#8211; Nanoscale Distance Measurements in EPR and Fluorescence Spectroscopies (August 28-29, 2018)&#8221;<\/span><\/a><\/p>\n","protected":false},"author":4475,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0},"categories":[65,61],"tags":[],"_links":{"self":[{"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/posts\/1661"}],"collection":[{"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/users\/4475"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/comments?post=1661"}],"version-history":[{"count":9,"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/posts\/1661\/revisions"}],"predecessor-version":[{"id":6319,"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/posts\/1661\/revisions\/6319"}],"wp:attachment":[{"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/media?parent=1661"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/categories?post=1661"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/irtgpolymers\/wp-json\/wp\/v2\/tags?post=1661"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}