{"id":163,"date":"2019-04-30T14:08:07","date_gmt":"2019-04-30T12:08:07","guid":{"rendered":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/?page_id=163"},"modified":"2020-04-30T20:10:03","modified_gmt":"2020-04-30T18:10:03","slug":"cryo-electron-microscopy","status":"publish","type":"page","link":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/research\/cryo-electron-microscopy\/","title":{"rendered":"Cryo-Electron Microscopy"},"content":{"rendered":"

Cryo-Electron Microscopy has revolutionized the field of high-resolution structural biology, and Dubochet, Frank and Henderson were awarded the Nobel Prize in Chemistry of 2017 due to their contributions for such an amazing development. Our laboratory was trained in EMBL-Heidelberg, where vitrification was invented by Jacques Dubochet. The Laboratory has high-end infrastructure of cryo-electron microscopes and associated sample preparation equipment.<\/p>\n

The JEOL JEM-3200FSC<\/h5>\n

\"\"<\/a>\"\"<\/a>Our JEOL microscope is equipped with Gatan Digital Micrograph and currently, JADAS for automated image acquisition is being installed. In the near future, SerialEM will also become available at the microscope. We use it for high-end data acquisition of selected samples having been screened at the Glacios micrososcope.<\/p>\n

Characteristics - JEOL JEM-3200FSC:<\/strong>\n300 kV cryo-TEM\nField emission source\nmotorized apertures and intuitive control \nloading up to 3 samples simultaneously\nK2 direct electron detector for high-end image acquisition\nCCD camera for sample screening and alignments\nin-column energy filter integrated into the electron optics\nliquid He- or LN2- cooled side-entry goniometer \n<\/pre>\n
<\/h5>\n
The Thermofischer FEI Glacios<\/h5>\n
\"\"<\/a>Our Glacios microscope is used for screening of samples under room-temperature and cryogenic conditions. We will be upgrading this machine with a phase plate, a direct electron detector, microED technology, and computational infrastructure.<\/p>\n
Characteristics - Thermo FEI Glacios:<\/strong>\n200 kV cryo-TEM\nField emission source\nmotorized apertures and intuitive control \nloading up to 12 samples simultaneously\nminimal contamination rates\nCMOS camera for sample screening and alignments<\/pre>\n
\"\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"
Cryo-Electron Microscopy has revolutionized the field of high-resolution structural biology, and Dubochet, Frank and Henderson were awarded the Nobel Prize in Chemistry of 2017 due to their contributions for such an amazing development. Our laboratory was trained in EMBL-Heidelberg, where Continue reading   Cryo-Electron Microscopy<\/span>→<\/span><\/a><\/p>\n","protected":false},"author":4167,"featured_media":0,"parent":27,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0},"_links":{"self":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/pages\/163"}],"collection":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/users\/4167"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/comments?post=163"}],"version-history":[{"count":9,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/pages\/163\/revisions"}],"predecessor-version":[{"id":380,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/pages\/163\/revisions\/380"}],"up":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/pages\/27"}],"wp:attachment":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/media?parent=163"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}