{"id":230,"date":"2019-05-22T15:31:00","date_gmt":"2019-05-22T13:31:00","guid":{"rendered":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/?p=230"},"modified":"2019-05-22T15:31:00","modified_gmt":"2019-05-22T13:31:00","slug":"defocus-estimation-currently-working-320-images-overnight","status":"publish","type":"post","link":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/2019\/05\/defocus-estimation-currently-working-320-images-overnight\/","title":{"rendered":"Defocus estimation currently working, 320 images overnight!"},"content":{"rendered":"<p>We have acquired overnight cryo-EM data of apoferritin, and calculations are in agreement with data acquisition in EPU. These days, we are optimizing vitrification of standard protein samples for a high-end data acquisition at the microscope; An image from Warp (http:\/\/www.warpem.com\/) is shown below, where the defocus estimation highly correlates with the actual defocus series implemented for data acquisition.<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/files\/2019\/05\/Warp-stream.png\"><img loading=\"lazy\" class=\"aligncenter size-large wp-image-231\" src=\"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/files\/2019\/05\/Warp-stream-1024x640.png\" alt=\"\" width=\"530\" height=\"331\" srcset=\"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/files\/2019\/05\/Warp-stream-1024x640.png 1024w, https:\/\/blogs.urz.uni-halle.de\/kastritislab\/files\/2019\/05\/Warp-stream-300x188.png 300w, https:\/\/blogs.urz.uni-halle.de\/kastritislab\/files\/2019\/05\/Warp-stream-768x480.png 768w, https:\/\/blogs.urz.uni-halle.de\/kastritislab\/files\/2019\/05\/Warp-stream.png 1920w\" sizes=\"(max-width: 530px) 100vw, 530px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We have acquired overnight cryo-EM data of apoferritin, and calculations are in agreement with data acquisition in EPU. These days, we are optimizing vitrification of standard protein samples for a high-end data acquisition at the microscope; An image from Warp <a class=\"more-link\" href=\"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/2019\/05\/defocus-estimation-currently-working-320-images-overnight\/\">Continue reading <span class=\"screen-reader-text\">  Defocus estimation currently working, 320 images overnight!<\/span><span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":4167,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0},"categories":[28],"tags":[7,48,33,49,47],"_links":{"self":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/posts\/230"}],"collection":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/users\/4167"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/comments?post=230"}],"version-history":[{"count":1,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/posts\/230\/revisions"}],"predecessor-version":[{"id":232,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/posts\/230\/revisions\/232"}],"wp:attachment":[{"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/media?parent=230"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/categories?post=230"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/kastritislab\/wp-json\/wp\/v2\/tags?post=230"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}