{"id":11,"date":"2017-05-05T08:36:49","date_gmt":"2017-05-05T06:36:49","guid":{"rendered":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/?page_id=11"},"modified":"2019-01-09T11:13:38","modified_gmt":"2019-01-09T10:13:38","slug":"single-molecule-fluorescence-spectroscopy","status":"publish","type":"page","link":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/single-molecule-fluorescence-spectroscopy\/","title":{"rendered":"Fluorescence Correlation Spectroscopy"},"content":{"rendered":"<p>Single-molecule fluorescence spectroscopy is a versatile tool to study single-particle properties in complex environments. We use single-molecule fluorescence correlation spectroscopy to study protein interactions and protein dynamics in solutions.<\/p>\n<h6>Example:<\/h6>\n<p>We are interested to find out how short- and far-range interactions between proteins affect the local dynamics of proteins in solutions.\u00a0 Hence, we studied the concentration dependence of the rotational (short-range) and translational (long-range) diffusion of diffeent proteins.\u00a0 High concentrations up to 20 vol% can be used to mimic complex environments and to study possible effects of a dynamic confinement.<\/p>\n<h6>Principle:<\/h6>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-117\" src=\"https:\/\/blogs.urz.uni-halle.de\/mariaott\/files\/2017\/05\/Picture1-1.gif\" alt=\"\" width=\"379\" height=\"562\" \/><\/p>\n<p><a href=\"https:\/\/blogs.urz.uni-halle.de\/mariaott\/methods\/\">&lt;Methods<\/a> <a href=\"https:\/\/blogs.urz.uni-halle.de\/mariaott\/\">&lt;&lt;Home<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Single-molecule fluorescence spectroscopy is a versatile tool to study single-particle properties in complex environments. We use single-molecule fluorescence correlation spectroscopy to study protein interactions and protein dynamics in solutions. Example: We are interested to find out how short- and far-range interactions between proteins affect the local dynamics of proteins in solutions.\u00a0 Hence, we studied the &hellip; <a href=\"https:\/\/blogs.urz.uni-halle.de\/mariaott\/single-molecule-fluorescence-spectroscopy\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Fluorescence Correlation Spectroscopy<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3032,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/pages\/11"}],"collection":[{"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/users\/3032"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/comments?post=11"}],"version-history":[{"count":7,"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/pages\/11\/revisions"}],"predecessor-version":[{"id":334,"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/pages\/11\/revisions\/334"}],"wp:attachment":[{"href":"https:\/\/blogs.urz.uni-halle.de\/mariaott\/wp-json\/wp\/v2\/media?parent=11"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}