Example<\/h6>\n
Scattering intensity of lysozyme at different concentrations:<\/p>\n
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Roos M, Ott M,<\/strong>\u00a0 <\/span>Hofmann M,\u00a0 <\/span>Link S, R\u00f6ssler E, Balbach J, Krushelnitsky A,\u00a0 <\/span>and K. Saalw\u00e4chter.\u00a0Coupling and Decoupling of Rotational and Translational Diffusion of Proteins under Crowding Conditions.<\/strong> J.\u00a0Am. Chem. Soc.<\/em> 138,10365\u201310372<\/a>\u00a0(2016)<\/p>\n <Methods<\/a> <<Home<\/a><\/p>\n X-ray diffraction can be used for determining the atomic or molecular structure of crystals or crystal-like samples. Relative distances and absolute positions of the first neighboring atoms can be deduced from the scattering intensity and scattering directions of the X-rays deviated by the atomic electronic densities.\u00a0X-ray scattering of amyloid fibrils results in a typical os-called cross-beta structure \u00a0reveal\u00a0a typical cross-\u03b2 structure with \u03b2-sheets arranged in parallel to the fibril axis and their constituent \u03b2-strands perpendicular to the fibril axis. \u00a0We investigated whether the interaction of fluorophores with the peptide has any impact to the fibrillar structure.\u00a0We found that the common reflection for the distances of the \u03b2-strands (4.75\u00c5) indicates very similar, if not identical, nuclei. With other words, the fluorophores did not affect the basic unit cell of the nuclei, as this structure would propagate during growth. However, the lateral order of the fibrillar bundles was modified, which is depicted by the dye-dependent differences of the other peak at 10 \u00c5.<\/p>\n <Methods<\/a> <<Home<\/a><\/p>\n","protected":false},"excerpt":{"rendered":" X-ray scattering of protein solutions We perform in-house X-ray scattering experiments in the q-range of 0.02-29 nm-1. The wide q-range is realized by varying the sample to detector distance of the 2D-detector. The sample stage contains several capillaries, which can be cooled or heated between -20\u00b0C and 180\u00b0C. Example Scattering intensity of lysozyme at different … Continue reading Bio SAXS \/ WAXS<\/span>
\nX-ray scattering of amyloid fibrils<\/h4>\n
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J. W\u00e4gele, S. De Sio, B. Voigt, J. Balbach, M. Ott.<\/b> How fluorescent tags modify oligomer size distributions of the Alzheimer-peptide.<\/strong> Biophys. J<\/em>. 116,\u00a0227-238<\/a>\u00a0(2019)<\/p>\n