Methods
The core facility offers a wide range of methods for light and electron microscopy, especially for tissue and cell preparation.
- classical with aldehydes for EM applications
- specialised fixation methods for light microscopy
- Cryofixation:
- Plunge freezing
- Propan-Jet freezing
- High-Pressure freezing
- Freeze substitution
- Freeze fracture, freeze etching
- Epoxy resins
- Methyl acrylates
- Polyethylene glycol mixtures
- Paraffin waxes and other
- Preparation of thin- and ultrathin sections
(including frozen sections)
- Light microscopy
- Brightfield
- Phase contrast
- Interference contrast
- Scanning electron microscopy
- Transmission electron microscopy
Histochemical staining
Immunological detection of biologically active substances
Immunofluorescence
Enzyme-coupled immunolabeling
Immunogold techniques
Cell- and tissue-specific localization of specific nucleic acids
(in situ hybridization)
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