Light Microscopes

Cryo-Confocal Fluorescence Microscope LSM 900 for Correlative Light and Electron Microscopy (CLEM)

Location: External: Charles-Tanford-Protein Center, Bacia Lab
www.chemie.uni-halle.de/bereiche_der_chemie/physikalische_chemie/prof_bacia/

Contact:  caroline.haupt@chemie.uni-halle.de
Category: Confocal, Cryo-Stage
Microscopy Stand: Upright stand, motorized z-focus
Stage: Motorized xy stage for room temperature, Linkam Cryo-Stage CMS196 with LN2 for EM grids

Laser lines: 405 nm, 488 nm, 561 nm, 640 nm
Transmitted illumination: LED
Fluorescence illumination: HXP 120 V
Detection:

• Axiocam 506 mono
• T-PMT
• Confocal, Airyscan 2 (optimized for 100× magnification), 32 channel GaAsP

Suitable for Specimen:  Cryo-fixed and room temperature on EM grids, fixed samples with # 1.5 coverglass

Objectives:

• 5×, 0,16 air, EC Plan-Neofluar 
• 50×, 0,6 air, DIC, LD C Epiplan-Apochromat
• 63×, 1,2 water, C-Apochromat
• 63×, 1,4 oil, DIC, Plan-Apochromat
• 100×, 0,75 air, DIC, LD C Epiplan-Neofluar

Condensor: 0,9 air, DIC
Filter sets: 49 (DAPI), 38 (GFP), 45 (Texas Red)

Please inquire for objectives, filter sets and condensor needed.