Light Microscopes
Cryo-Confocal Fluorescence Microscope Zeiss LSM 900 for Correlative Light and Electron Microscopy (CLEM)
Location: External: Charles-Tanford-Protein Center, Bacia Lab
www.chemie.uni-halle.de/bereiche_der_chemie/physikalische_chemie/prof_bacia/
Contact: caroline.haupt@chemie.uni-halle.de
Category: Confocal, Cryo-Stage
Microscopy Stand: Upright stand, motorized z-focus
Stage: Motorized xy stage for room temperature, Linkam Cryo-Stage CMS196 with LN2 for EM grids
Laser lines: 405 nm, 488 nm, 561 nm, 640 nm
Transmitted illumination: LED
Fluorescence illumination: HXP 120 V
Detection:
- Axiocam 506 mono
- T-PMT
- Confocal, Airyscan 2 (optimized for 100× magnification), 32 channel GaAsP
Suitable for Specimen: Cryo-fixed and room temperature on EM grids, fixed samples with # 1.5 coverglass
Objectives:
- 5×, 0,16 air, EC Plan-Neofluar
- 50×, 0,6 air, DIC, LD C Epiplan-Apochromat
- 63×, 1,2 water, C-Apochromat
- 63×, 1,4 oil, DIC, Plan-Apochromat
- 100×, 0,75 air, DIC, LD C Epiplan-Neofluar
Condensor: 0,9 air, DIC
Filter sets: 49 (DAPI), 38 (GFP), 45 (Texas Red)
Please inquire for objectives, filter sets and condensor needed.
