Cryo-Electron Microscopy has revolutionized the field of high-resolution structural biology, and Dubochet, Frank and Henderson were awarded the Nobel Prize in Chemistry of 2017 due to their contributions for such an amazing development. Our laboratory was trained in EMBL-Heidelberg, where vitrification was invented by Jacques Dubochet. The Laboratory has high-end infrastructure of cryo-electron microscopes and associated sample preparation equipment.
The JEOL JEM-3200FSC
Our JEOL microscope is equipped with Gatan Digital Micrograph and currently, JADAS for automated image acquisition is being installed. In the near future, SerialEM will also become available at the microscope. We use it for high-end data acquisition of selected samples having been screened at the Glacios micrososcope.
Characteristics - JEOL JEM-3200FSC: 300 kV cryo-TEM Field emission source motorized apertures and intuitive control loading up to 3 samples simultaneously K2 direct electron detector for high-end image acquisition CCD camera for sample screening and alignments in-column energy filter integrated into the electron optics liquid He- or LN2- cooled side-entry goniometer
The Thermofischer FEI Glacios
Our Glacios microscope is used for screening of samples under room-temperature and cryogenic conditions. We will be upgrading this machine with a phase plate, a direct electron detector, microED technology, and computational infrastructure.
Characteristics - Thermo FEI Glacios: 200 kV cryo-TEM Field emission source motorized apertures and intuitive control loading up to 12 samples simultaneously minimal contamination rates CMOS camera for sample screening and alignments