Single Molecule Fluorescence Spectroscopy

Single-molecule fluorescence spectroscopy is a versatile tool to study single-particle properties in complex environments. We use single-molecule fluorescence spectroscopy to study protein interactions and protein dynamics in solutions.

Example: We are interested to find out how short- and far-range interactions between proteins affect the local dynamics of proteins in solutions.  Hence, we studied the concentration dependence of the rotational (short-range) and translational (long-range) diffusion of diffeent proteins.  High concentrations up to 20 vol% can be used to mimic complex environments and to study possible effects of a dynamic confinement.

Principle:

Science between polymers and biophysics