Defocus estimation currently working, 320 images overnight!

We have acquired overnight cryo-EM data of apoferritin, and calculations are in agreement with data acquisition in EPU. These days, we are optimizing vitrification of standard protein samples for a high-end data acquisition at the microscope; An image from Warp ( is shown below, where the defocus estimation highly correlates with the actual defocus series implemented for data acquisition.


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