Glacios – Kastritis Laboratory for Biomolecular Research

Our paper on the 2.7 Å structure of apoferritin with the Glacios and the Falcon 3EC is available as a preprint!

Posted on 26/08/2019 by Panagiotis Kastritis — No Comments ↓

Our paper is available as a pre-print in Biorxiv (https://www.biorxiv.org/content/10.1101/738724v1). In this work, we have determined the atomic structure of mouse apoferritin using the Glacios microscope. Its difference comparedd to other 200 keV microscope is its compact design, allowing the Continue reading Our paper on the 2.7 Å structure of apoferritin with the Glacios and the Falcon 3EC is available as a preprint!→

Vitrified apoferritin in Halle – first overnight acquisition is complete!

Posted on 23/05/2019 by Panagiotis Kastritis — No Comments ↓

We have successfully vitrified apoferritin in own Vitrobot Mark 4. Loading the microscope and starting EPU yesterday evening ended up with an acquisition of ~300 high-resolution images from a single gridsquare! First of all, we are able to demonstrate the Continue reading Vitrified apoferritin in Halle – first overnight acquisition is complete!→

Defocus estimation currently working, 320 images overnight!

Posted on 22/05/2019 by Panagiotis Kastritis — No Comments ↓

We have acquired overnight cryo-EM data of apoferritin, and calculations are in agreement with data acquisition in EPU. These days, we are optimizing vitrification of standard protein samples for a high-end data acquisition at the microscope; An image from Warp Continue reading Defocus estimation currently working, 320 images overnight!→

a sneak peek into Halle automated acquisition

Posted on 21/05/2019 by Panagiotis Kastritis — No Comments ↓

Thanks to Warp (http://www.warpem.com/warp/), an amazing on-the-fly image processing suite for cryo-EM data, we are able not only to acquire beautiful apoferritin images with EPU, but also estimate the quality of our image acquisition on the fly!

Getting EPU up and working…

Posted on 07/05/2019 by Panagiotis Kastritis — No Comments ↓

Already acquiring successful grid Atlases, seems the software is robust and communicates well with the Glacios! On the left of the image, you can see the gridmapping, on the right the feature that was used to calibrate image shifts!