imaging – Kastritis Laboratory for Biomolecular Research

Vitrified apoferritin in Halle – first overnight acquisition is complete!

Posted on 23/05/2019 by Panagiotis Kastritis — No Comments ↓

We have successfully vitrified apoferritin in own Vitrobot Mark 4. Loading the microscope and starting EPU yesterday evening ended up with an acquisition of ~300 high-resolution images from a single gridsquare! First of all, we are able to demonstrate the Continue reading Vitrified apoferritin in Halle – first overnight acquisition is complete!→

Defocus estimation currently working, 320 images overnight!

Posted on 22/05/2019 by Panagiotis Kastritis — 1 Comment ↓

We have acquired overnight cryo-EM data of apoferritin, and calculations are in agreement with data acquisition in EPU. These days, we are optimizing vitrification of standard protein samples for a high-end data acquisition at the microscope; An image from Warp Continue reading Defocus estimation currently working, 320 images overnight!→