Kastritis Laboratory for Biomolecular Research
Cryo-EM, Computation and Metabolons
We are very proud for our cover for Biol Chem, July issue – it is currently online, and advertised on the main page of the journal. A high-resolution pdf file with explanation of our cover can be found on the Continue reading The cover for Biol Chem – Structural Biology of Native Cell Extracts
The software of Scheres, Relion 3 (https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page), we have it on our cluster and on the iMACs! For the iMacs, code can be found below, thanks to our scientific consultant and external collaborator, Koen M. Visscher: First install the developer Continue reading Installation of Relion 3 on our MacOS!
We have successfully vitrified apoferritin in own Vitrobot Mark 4. Loading the microscope and starting EPU yesterday evening ended up with an acquisition of ~300 high-resolution images from a single gridsquare! First of all, we are able to demonstrate the Continue reading Vitrified apoferritin in Halle – first overnight acquisition is complete!
Our accepted manuscript “Integrative biology of native cell extracts: a new era for structural characterization of life processes” by Kyrilis et al is currently online and open access – you can find it here. You can also view the PDF Continue reading Publication of the Kastritis Lab – We got the cover of the Biol Chem (July issue)!
We have acquired overnight cryo-EM data of apoferritin, and calculations are in agreement with data acquisition in EPU. These days, we are optimizing vitrification of standard protein samples for a high-end data acquisition at the microscope; An image from Warp Continue reading Defocus estimation currently working, 320 images overnight!