Kastritis Laboratory for Biomolecular Research
Cryo-EM, Computation and Metabolons
In a collaborative effort with the Sinz lab, our Post-doc Christian led a project on the utilization of AI-derived protein modeling based on mass spectrometry data, in order to interpret unknown cryo-EM maps from within native cell extracts! This type Continue reading AlphaFold2 and Proteomics data help interpret native cell extracts
Our PhD student Toni wrote a very nice opinion piece on the latest work published by our collaborator Francis O’Reilly et al. where they comment on how integration of cross-linking data, fractionation, AI-based modeling, biochemical experiments and data mining can Continue reading AlphaFold2 + In-cell cross-linking = New PPI Insights!
Our work on how cryo-EM and AI can be combined to structurally characterize protein community members from native cell extracts is featured on the cover of the April Issue of Structure! We want to thank again our lab members and Continue reading On the cover of Structure
We welcome 2022 with our latest publication on Structure, on how the latest advances in AI-driven protein model prediction can help us in the interpretation of complex cryo-EM data from native cell extracts! We are very excited about the impact Continue reading Cryo-EM combined with AI to advance our understanding of native cell extracts!
Our latest work on the structure and function of the native Pyruvate Dehydrogenase Complex Core is now online, showing once again the power of our lab’s integrative approach! Cryo-EM, macromolecular docking and molecular dynamics simulations are combined to provide insights Continue reading Structural insights into a metabolon-embedded transacetylase reaction powered by Cryo-EM!