NMR spectroscopy for biomolecular and materials characterization (September 6/7, 2017)

Aims

This module addressed doctoral students in chemistry, physics, biophysics, and related fields without much prior knowledge in NMR spectroscopy. It introduced the basic theoretical and technical concepts necessary for an understanding of data/spectrum acquisition in pulsed NMR and the information content, and focused on a few specific experimental techniques to study dynamics in polymer and biopolymer systems.

Contents

Each day of the two-day program started with a series of lectures in the morning, followed by hands-on experiments on low-field NMR spectrometers, and demonstrations on high-field spectrometers, during the afternoon.

Lectures:

• basic principles of pulsed NMR: time evolution of magnetization (precession) and Fourier transformation, NMR interactions and spectral information content
• technical aspects of pulsed NMR: radio-frequency pulses, frequency mixing (=rotating frame), phase-sensitive detection, digitization
• influence of molecular structure and dynamics on NMR observables, applications to polymers
• use of pulsed field gradients for imaging and diffusion, applications to the measurement of diffusion of polymers to study molecular weight and conformation

Hands-on lab course:

• basic pulsed NMR: time-domain experiments and Fourier transformation, determination of relaxation times
• study of phase structure and dynamics in inhomogeneous (phase-separated) polymers, biopolymers or food systems by time-domain low-field NMR: bring your own sample!
• study of macro(molecular) diffusion by pulsed-gradient NMR, applications to protein solutions

Literature

• E. Fukushima, S. B. W. Roeder: Experimental Pulse NMR: A Nuts and Bolts Approach. Westview Press, Boulder 1993
• K. Saalwächter, D. Reichert: Magnetic Resonance: Polymer Applications of NMR. In: Encyclodedia of Spectroscopy & Spectrometry, 2nd edition, vol. 3, pp. 2221-2236. Editors: J. Lindon, G. Tranter, D. Koppenaal. Elsevier, Oxford 2010. (Abstract)

Terms and Applications

Date: September6-7, 2017
Time: 9.15-5pm
Venue: seminar room, NMR building, Betty-Heimann-Str. 7, 06120 Halle
Group size: maximal 12
Language: English

Instructor(s)

Kay Saalwächter, Alexey Krushelnitsky, Günter Hempel

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