Interactions of single polymer chains and Amyloid fibrils in a thermophoretic trap
by Stephan Sydow
and
Ion Soft-landing: Introduction in Preparative Mass Spectrometry to Generate Polymers
by Sebastian Kawa
date: October 19, 15.00
hybrid seminar: Open Space wunderbar (Gießerstr. 18, Leipzig-Plagwitz) + zoom
chair: Marthinus
Abstracts
Interactions of single polymer chains and Amyloid fibrils in a thermophoretic trap
by Stephan Sydow
The understanding of amyloid fibril formation as a fundamental process of protein aggregation is hampered by the heterogeneity of the ensemble that is commonly studied. It is in particular difficult to extract information on the detailed processes, like primary and secondary nucleation as well as varying growth rates from an ensemble average over different species. The aim of this project is to contribute with microscopic studies of the fibril formation and growth to the overall picture of amyloid formation. We aim to constrain single seed fibrils spatially to directly image in real time their growth, branching or fragmentation and its dependence on the protein structure and other ingredients modifying the fibril growth. This shall provide a microscopic foundation for the kinetic models in the literature.
Ion Soft-landing: Introduction in Preparative Mass Spectrometry to Generate Polymers
by Sebastian Kawa
Electrospray ionization mass spectrometry (ESI-MS) is widely used in chemical analysis. Inside the mass spectrometer ions can be excited to generate highly reactive fragment ions which are not amenable in the condensed phase. Ion soft-landing allows the deposition of mass selected gaseous ions on surfaces and therefore enables their utilization for preparative applications. Our aim is to deposit highly reactive fragment ions to initialize polymerization of co-deposited monomers. This approach may lead to polymers with unique properties that cannot be generated by conventional methods. The instruments used for ion soft-landing and analysis of the samples (preparative and analytical mass spectrometry) are introduced in the presentation.
