Doctoral students seminar (November 29, 2022)

Binding Reactive Fragment Ions to Peptides
by Sebastian Kawa

date: November 29, 14.30
hybrid seminar: Aula, room 331 (first floor), Linnéstr. 5, Leipzig + zoom

Binding Reactive Fragment Ions to Peptides

Sebastian Kawa

Electrospray ionization mass spectrometry (ESI-MS) is widely used in chemical analysis. Inside the mass spectrometer ions can be excited to generate highly reactive fragment ions which are not amenable in the condensed phase. Ion soft-landing allows the deposition of mass selected gaseous ions on surfaces and therefore enables their utilization for preparative applications. Our aim is to deposit highly reactive fragment ions and bind them to co-deposited peptides. This approach may lead to functionalized peptides with unique properties that cannot be generated by conventional methods. The deposited surfaces are analyzed with liquid extraction surface analysis (LESA) to obtain information about the reaction products. In this presentation the reaction behavior of the fragment ions [B12I8SCN] and [B12I11] when co-deposited with leucylproline is compared and the binding mechanism is investigated.