[BEAM] ATM: NMR spectroscopy for biomolecular and materials characterization (March, 2023)

Aims

This module addresses doctoral students in chemistry, physics, biophysics, and related fields without much prior knowledge in NMR spectroscopy. It introduces the basic theoretical and technical concepts necessary for an understanding of data/spectrum acquisition in pulsed NMR and the information content, and focuses on a few specific experimental techniques to study dynamics in polymer and biopolymer systems.

Schedule

Day 1

  1. Basics: spins polarization, pulsed NMR, precession, FID
    signal and sensitivity, relaxation times
  2. Technical details: function of a pulsed NMR spectrometer
  3. Data acquisition and processing: Fourier trans-formation,
    phases and offsets, sampling issues
  4. Afternoon lab: learn to apply the basics

Day 2

  1. Solid-state NMR: line broadening by anisotropic interactions,dipole-dipole couplings, dynamic averaging effects
  2. (Pulsed) field gradients: imaging and diffusion
    7 Afternoon lab: play with soft matter samples

Afternoon lab:
play with soft matter samples

  • qualitative molecular mobility by 1H low-field NMR
  • learn how to use and calibrate gradients (1H low field)
  • diffusion measurement in protein solutions (1H high field)
Literature
  • J. Keeler, Understanding NMR Spectroscopy, John Wiley & Sons, Chichester 2010 (+ short lecture version 2002/2004)
  • M. H. Levitt, “Spin Dynamics”, John Wiley & Sons, Chichester 2008
  • E. Fukushima, S. B. W. Roeder, “Experimental Pulse NMR”, Westview Press, Boulder 1981
  • K. Schmidt-Rohr, H. W. Spiess, “Multidimensional Solid-State NMR and Polymers”, Academic Press, San Diego 1994
Lecturers

Kay Saalwächter

Date

either March 9/10, 2023 or March 13/14, 2023

Registration

Please contact Imme Sakwa-Waltz in case you plan to join this ATM!