Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers
The formation of amyloid fibrils is considered to be one of the main causes for many neurodegenerative diseases, such as Alzheimer’s, Parkinson’s or Huntington’s disease . Current knowledge suggests that amyloid-aggregation represents a nucleation-dependent aggregation process in vitro, where a sigmoidal growth phase follows an induction period. Here, we studied the fibrillation of amyloid β 1-40 (Aβ40) in the presence of thermoresponsive polymers, expected to alter the Aβ40 fibrillation kinetics due to their lower critical solution behavior [2-6]. To probe the influence of molecular weight and the end groups of the polymer on its lower critical solution temperature (LCST), also considering its concentration dependence in the presence of buffer-salts needed for the aggregation studies of the amyloids poly(methoxy di(ethylene glycol)acrylates) with different LCSTs were synthesized. Mixtures and a conjugate of Aβ40 with these polymers in varying concentrations were studied via time-dependent measurements of the thioflavin T (ThT) fluorescence. The studies revealed that amyloid fibrillation was accelerated, accompanied by a reduction of the lag phase of Aβ40 fibrillation in the presence of the poly(methoxy di(ethylene glycol)acrylates) .
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Location: Von-Danckelmann-Platz 3, SR 1.03 Time: 3.30pm