Nanoscale Distance Measurements in EPR and Fluorescence Spectroscopies (August 28-29, 2018)

Aims

This module is suitable for doctoral students in chemistry, physics, biophysics, and related fields. Some background in quantum mechanics is advisable but no direct prerequisite. It introduces the basic theoretical and technical concepts necessary for an understanding of electron paramagnetic resonance and fluorescence in general and especially emphasises the EPR and fluorescence methods for measuring distances on the nanometer scale.

Contents

Each day of the two-day program starts with a series of lectures in the morning covering the theoretical knowledge by basic lectures and the discussion of applications and problems. It is followed by hands-on experiments on instructive examples during the afternoon.

Lectures:

  • basic principles of magnetic resonance, technical spectrometer set-up, chemistry of spin labeling and spin probing, pulse EPR spectroscopy, data processing/analysis, applications to (bio-)polymers
  • basic principles of single-molecule and time-resolved fluorescence spectroscopy and single-molecule FRET, including methods of fluorescence labelling, microscope set-up, single-molecule detection and data analysis

Hands-on lab course:

  • Sample preparation and CW EPR spectroscopy; guided experiments in pulse EPR spectroscopy; applications and analysis for distance measurements
  • guided single-molecule and time-resolved FRET experiments of proteins in solution, data analysis for intramolecular distance measurements

Recommendations for literature

  • A. Weil, J. R. Bolton, Electron Paramagnetic Resonance: elementary theory and practical applications. John Wiley & Sons Inc.: Hoboken, New Jersey, 2007.
  • Schweiger, G. Jeschke, Principles of pulse electron paramagnetic resonance. Oxford University Press Inc.: Oxford, 2001.
  • Jeschke, Emerging Top Life Sci 2018, 2, 9–18
  • J. N. Junk, H. W. Spiess, D. Hinderberger, Angew Chem Int Ed 2010,49, 8755–8759.
  • Lakowicz, J.R. Principles of fluorescence spectroscopy, Springer, 2006
  • Jammeson D.M.. Perspectives on Fluorescence, Springer, 2016
  • Dimura M., et al. Quantitative FRET studies and integrative modeling unravel the structure and dynamics of biomolecular systems, Curt Opin Struct Biol 40: 163-185 (2016)

Terms and Applications

Fluorescence at August 28, 9:00 – 16:00
Venue: Halle (Saale), details to be announced

EPR at August 29, 9:00 – 16:00
Venue: Halle (Saale), details to be announced

Group size: maximal 10
Language: English

Instructor(s)

Dr. Maria Ott,  Prof. Dr. Darisuh Hinderberger

Registration

Membership status
iRTG memberiGS Functional Polymersexternal

Please contact sfb-trr-102-office(at)physik.uni-halle.de in case of questions.

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